Identification of sucrase activity in cell suspension and culture medium of melon
| Autor: | J. Stano, K. Mičieta, A. Barth, M. Valšíková, M. Fulmeková, P. Matejka, M. Varadínová |
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| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: | |
| Zdroj: | Horticultural Science, Vol 32, Iss 3, Pp 104-107 (2005) |
| Druh dokumentu: | article |
| ISSN: | 0862-867X 1805-9333 |
| DOI: | 10.17221/3774-HORTSCI |
| Popis: | The activity of (soluble acid) sucrase was detected in a culture medium of the cell suspension culture of watermelon (Citrullus vulgaris L.). A simple and rapid procedure for the identification and determination of extracellular sucrase from a culture medium of watermelon cell suspension cultures is described. Sucrose was used as a substrate for the determination of extracellular and intracellular activities of the enzyme. Intracellular activity was estimated from the cell suspension. The results show a 91.5-92.0% intracellular and 8.0-8.5% extracellular distribution of sucrase activity. The described method enables to carry out a rapid, simple and specific detection of extracellular sucrase in plants. |
| Databáze: | Directory of Open Access Journals |
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