Fluid Dynamics Optimization of Microfluidic Diffusion Systems for Assessment of Transdermal Drug Delivery: An Experimental and Simulation Study

Autor: Dorottya Kocsis, Shanmugam Dhinakaran, Divyam Pandey, András József Laki, Mária Laki, Dániel Sztankovics, Miléna Lengyel, Judit Vrábel, Márton Bese Naszlady, Anna Sebestyén, Jeyaraj Ponmozhi, István Antal, Franciska Erdő
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Scientia Pharmaceutica, Vol 92, Iss 2, p 35 (2024)
Druh dokumentu: article
ISSN: 2218-0532
0036-8709
DOI: 10.3390/scipharm92020035
Popis: Organ-on-a-chip technologies show exponential growth driven by the need to reduce the number of experimental animals and develop physiologically relevant human models for testing drugs. In vitro, microfluidic devices should be carefully designed and fabricated to provide reliable tools for modeling physiological or pathological conditions and assessing, for example, drug delivery through biological barriers. The aim of the current study was to optimize the utilization of three existing skin-on-a-chip microfluidic diffusion chambers with various designs. For this, different perfusion flow rates were compared using cellulose acetate membrane, polyester membrane, excised rat skin, and acellular alginate scaffold in the chips. These diffusion platforms were integrated into a single-channel microfluidic diffusion chamber, a multi-channel chamber, and the LiveBox2 system. The experimental results revealed that the 40 µL/min flow rate resulted in the highest diffusion of the hydrophilic model formulation (2% caffeine cream) in each system. The single-channel setup was used for further analysis by computational fluid dynamics simulation. The visualization of shear stress and fluid velocity within the microchannel and the presentation of caffeine progression with the perfusion fluid were consistent with the measured data. These findings contribute to the development and effective application of microfluidic systems for penetration testing.
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje