| Autor: |
Masaki Kimura, Momoko Azuma, Ran-Ran Zhang, Wendy Thompson, Christopher N. Mayhew, Takanori Takebe |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
iScience, Vol 4, Iss , Pp 294-301 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2589-0042 |
| DOI: |
10.1016/j.isci.2018.05.007 |
| Popis: |
Summary: Radio frequency identification (RFID) is a cost-effective and durable method to trace and track individual objects in multiple contexts by wirelessly providing digital signals; RFID is thus widely used in many fields. Here, we implement this concept to biological tissues by producing a compact RFID chip-incorporated organoid (RiO). The 0.4 mm RFID chips are reproducibly integrated inside the self-assembling organoids from 10 different induced pluripotent stem cell (iPSC) lines from healthy and diseased donors. We use the digitalized RiO to conduct a phenotypic screen on a pool of RiO, followed by detection of each specific donor in situ. Our proof-of-principle experiments demonstrated that a severely steatotic phenotype could be identified by RFID chip reading and was specific to a genetic disorder of steatohepatitis. Given evolving advancements surrounding RFID technology, the digitalization principle outlined here will expand organoid medicine potential toward drug development, precision medicine, and transplant applications. : Cell Biology; Stem Cells Research; Bioengineering Subject Areas: Cell Biology, Stem Cells Research, Bioengineering |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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