Chromogenic Substrate Assay for Determining the Activity of Plasma Kallikrein
| Autor: | Praveen Papareddy, Martina Kalle, Artur Schmidtchen |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Bio-Protocol, Vol 4, Iss 19 (2014) |
| Druh dokumentu: | article |
| ISSN: | 2331-8325 |
| DOI: | 10.21769/BioProtoc.1246 |
| Popis: | The activation of the intrinsic pathway takes place at negatively charged surfaces, such as bacteria, and involves activation of cogulation Factor XII, which then leads to the activation of plasma kallikrein (PK) and coagulation Factor XI. To determine the PK activity on bacterial surfaces, bacteria were pre-incubated with peptides, followed by incubation with plasma, and the effect of peptide was recorded by measuring the PK activity. |
| Databáze: | Directory of Open Access Journals |
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