Zona pellucida treatment before CRISPR/Cas9‐mediated genome editing of porcine zygotes

Autor:	Zhao Namula, Maki Hirata, Quynh Anh Le, Qingyi Lin, Koki Takebayashi, Naoaki Yoshimura, Fuminori Tanihara, Chommanart Thongkittidilok, Takeshige Otoi
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	CRISPR electroporation genome editing porcine zygote zona pellucida Veterinary medicine SF600-1100
Zdroj:	Veterinary Medicine and Science, Vol 8, Iss 1, Pp 164-169 (2022)
Druh dokumentu:	article
ISSN:	2053-1095
DOI:	10.1002/vms3.659
Popis:	Abstract Background Increasing the permeability of the zona pellucida (ZP) of oocytes before CRISPR/Cas9 electroporation may improve the efficiency of gene editing; however, the effects of this approach on subsequent developmental processes are unclear. In this study, the effects of ZP treatment before electroporation on embryonic development and gene editing in porcine embryos were evaluated. Methods The ZP of zygotes was weakened or removed by exposure to 0.5% actinase E, followed by electroporation of the Cas9 protein with guide RNA targeting GGTA1. Results The blastocyst formation rate of ZP‐free zygotes after electroporation was significantly lower (p
Databáze:	Directory of Open Access Journals
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