Multiple soluble malate dehydrogenase of Geophagus brasiliensis (Cichlidae, Perciformes)
| Autor: | Aquino-Silva Maria Regina de, Schwantes Maria Luiza B., Schwantes Arno Rudi |
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| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | Genetics and Molecular Biology, Vol 21, Iss 4 (1998) |
| Druh dokumentu: | article |
| ISSN: | 1415-4757 1678-4685 |
| Popis: | A recent locus duplication hypothesis for sMDH-B* was proposed to explain the complex electrophoretic pattern of six bands detected for the soluble form of malate dehydrogenase (MDH, EC 1.1.1.37) in 84% of the Geophagus brasiliensis (Cichlidae, Perciformes) analyzed (AB1B2 individuals). Klebe's serial dilutions were carried out in skeletal muscle extracts. B1 and B2 subunits had the same visual end-points, reflecting a nondivergent pattern for these B-duplicated genes. Since there is no evidence of polyploidy in the Cichlidae family, MDH-B* loci must have evolved from regional gene duplication. Tissue specificities, thermostability and kinetic tests resulted in similar responses from both B-isoforms, in both sMDH phenotypes, suggesting that these more recently duplicated loci underwent the same regulatory gene action. Similar results obtained with the two sMDH phenotypes did not show any indication of a six-banded specimen adaptive advantage in subtropical regions. |
| Databáze: | Directory of Open Access Journals |
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