Gene transfection mediated by polyethyleneimine-polyethylene glycol nanocarrier prevents cisplatin-induced spiral ganglion cell damage
| Autor: | Guan-gui Chen, Min Mao, Li-zi Qiu, Qi-ming Liu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
tamoxifen
Src kinase PP2 trauma regeneration neuroprotection auranofin dextromethorphan rosiglitazone Alzheimer′s disease neuroinflammation neurodegeneration microglia astrocytes nerve regeneration spinal cord electroacupuncture therapy neural stem cells notch signaling pathway inflammation survival curve proliferation differentiation real-time quantitative PCR western blot assay neural regeneration superparamagnetic iron oxide magnetic guidance bone marrow mesenchymal stem cells spinal cord injury cell transplantation magnetic resonance image lumbar puncture spinal cord transection average combined score magnetic resonance imaging diffusion tensor imaging fractional anisotropy apparent diffusion coefficient fiber tractography peripheral nerve injury sciatic nerve hypothermia blood-nerve barrier Evans blue tracer neural degeneration polyethyleneimine-polyethylene glycol spiral ganglion cells X-linked inhibitor of apoptosis protein gene therapy nanocarrier cisplatin ototoxicity cochlea Neurology. Diseases of the nervous system RC346-429 |
| Zdroj: | Neural Regeneration Research, Vol 10, Iss 3, Pp 425-431 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1673-5374 |
| DOI: | 10.4103/1673-5374.153691 |
| Popis: | Polyethyleneimine-polyethylene glycol (PEI-PEG), a novel nanocarrier, has been used for transfection and gene therapy in a variety of cells. In our previous study, we successfully carried out PEI-PEG-mediated gene transfer in spiral ganglion cells. It remains unclear whether PEI-PEG could be used for gene therapy with X-linked inhibitor of apoptosis protein (XIAP) in the inner ear. In the present study, we performed PEI-PEG-mediated XIAP gene transfection in the cochlea of Sprague-Dawley rats, via scala tympani fenestration, before daily cisplatin injections. Auditory brainstem reflex tests demonstrated the protective effects of XIAP gene therapy on auditory function. Immunohistochemical staining revealed XIAP protein expression in the cytoplasm of cells in the spiral ganglion, the organ of Corti and the stria vascularis. Reverse transcription-PCR detected high levels of XIAP mRNA expression in the cochlea. The present findings suggest that PEI-PEG nanocarrier-mediated XIAP gene transfection results in XIAP expression in the cochlea, prevents damage to cochlear spiral ganglion cells, and protects hearing. |
| Databáze: | Directory of Open Access Journals |
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