Enhanced resistance to heat and fungal infection in transgenic Trichoderma via over-expressing the HSP70 gene

Autor: Yanhua Huang, Changfa Liu, Xuexue Huo, Xianzhi Lai, Wentao Zhu, Yongren Hao, Zehui Zheng, Kai Guo
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: AMB Express, Vol 14, Iss 1, Pp 1-16 (2024)
Druh dokumentu: article
ISSN: 2191-0855
DOI: 10.1186/s13568-024-01693-5
Popis: Abstract Heat stress is one of the major abiotic stresses affecting the growth, sporulation, colonization and survival of Trichoderma viride. This study aimed to gain a better insight into the underlying mechanism governing the heat stress response of T. viride Tv-1511. We analysed the transcriptomic changes of Tv-1511 under normal and heat stress conditions using RNA sequencing. We observed that Tv-1511 regulates the biosynthesis of secondary metabolites through a complex network of signalling pathways. Additionally, it significantly activates the anti-oxidant defence system, heat shock proteins and stress-response-related transcription factors in response to heat stress. TvHSP70 was identified as a key gene, and transgenic Tv-1511 overexpressing TvHSP70 (TvHSP70-OE) was generated. We conducted an integrated morphological, physiological and molecular analyses of the TvHSP70-OE and wild-type strains. We observed that TvHSP70 over-expression significantly triggered the growth, anti-oxidant capacity, anti-fungal activity and growth-promoting ability of Tv-1511. Regarding anti-oxidant capacity, TvHSP70 primarily up-regulated genes involved in enzymatic and non-enzymatic anti-oxidant systems. In terms of anti-fungal activity, TvHSP70 primarily activated genes involved in the synthesis of enediyne, anti-fungal and aminoglycoside antibiotics. This study provides a comparative analysis of the functional significance and molecular mechanisms of HSP70 in Trichoderma. These findings provide a valuable foundation for further analyses.
Databáze: Directory of Open Access Journals
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