Millisecond mix-and-quench crystallography (MMQX) enables time-resolved studies of PEPCK with remote data collection

Autor:	Jonathan A. Clinger, David W. Moreau, Matthew J. McLeod, Todd Holyoak, Robert E. Thorne
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	time-resolved crystallography enzymology protein structure structure determination x-ray crystallography enzyme mechanisms millisecond mix-and-quench crystallography protein dynamics structural biology Crystallography QD901-999
Zdroj:	IUCrJ, Vol 8, Iss 5, Pp 784-792 (2021)
Druh dokumentu:	article
ISSN:	2052-2525 20522525
DOI:	10.1107/S2052252521007053
Popis:	Time-resolved crystallography of biomolecules in action has advanced rapidly as methods for serial crystallography have improved, but the large number of crystals and the complex experimental infrastructure that are required remain serious obstacles to its widespread application. Here, millisecond mix-and-quench crystallography (MMQX) has been developed, which yields millisecond time-resolved data using far fewer crystals and routine remote synchrotron data collection. To demonstrate the capabilities of MMQX, the conversion of oxaloacetic acid to phosphoenolpyruvate by phosphoenolpyruvate carboxykinase (PEPCK) is observed with a time resolution of 40 ms. By lowering the entry barrier to time-resolved crystallography, MMQX should enable a broad expansion in structural studies of protein dynamics.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/08d4207bde694ed3ab91e4b1839b6b15 Zobrazit plný text záznamu View record in DOAJ