Conventional and Microfluidic Methods for the Detection of Nucleic Acid of SARS-CoV-2

Autor: Weidu Song, Taiyi Zhang, Huichao Lin, Yujing Yang, Gaozhen Zhao, Xiaowen Huang
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Micromachines, Vol 13, Iss 4, p 636 (2022)
Druh dokumentu: article
ISSN: 2072-666X
DOI: 10.3390/mi13040636
Popis: Nucleic acid testing (NAT) played a crucial role in containing the spread of SARS-CoV-2 during the epidemic. The gold standard technique, the quantitative real-time polymerase chain reaction (qRT-PCR) technique, is currently used by the government and medical boards to detect SARS-CoV-2. Due to the limitations of this technology, it is not capable of meeting the needs of large-scale rapid detection. To solve this problem, many new techniques for detecting nucleic acids of SARS-CoV-2 have been reported. Therefore, a review that systematically and comprehensively introduces and compares various detection technologies is needed. In this paper, we not only review the traditional NAT but also provide an overview of microfluidic-based NAT technologies and summarize and discuss the characteristics and development prospects of these techniques.
Databáze: Directory of Open Access Journals