Impact of ultrasound on pumpkin seed protein concentrate hydrolysis: Effects on Alcalase, protein, and assisted reaction

Autor: Ana Flávia Coelho Pacheco, Leticia Bruni de Souza, Paulo Henrique Costa Paiva, Carini Aparecida Lelis, Erica Nascif Rufino Vieira, Alline Artigiani Lima Tribst, Bruno Ricardo de Castro Leite Júnior
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Applied Food Research, Vol 3, Iss 1, Pp 100281- (2023)
Druh dokumentu: article
ISSN: 2772-5022
DOI: 10.1016/j.afres.2023.100281
Popis: Ultrasound (40 kHz, 23.8 W/L) was applied (pH 7.5, 25 – 60 °C, up to 300 min) to Alcalase (protease) and pumpkin seed protein concentrate (substrate) as a pre-process or during proteolysis aiming to improve the hydrolysis rate, the final concentration of hydrolysates, and its solubility and in vitro antioxidant activity. The pre-process applied in enzyme and substrate increased the relative activity of the enzyme by 10.3 and 50.1%, respectively (p
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