Decoupling tRNA promoter and processing activities enables specific Pol-II Cas9 guide RNA expression

Autor: David J. H. F. Knapp, Yale S. Michaels, Max Jamilly, Quentin R. V. Ferry, Hector Barbosa, Thomas A. Milne, Tudor A. Fulga
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Nature Communications, Vol 10, Iss 1, Pp 1-14 (2019)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-019-09148-3
Popis: The utility of CRISPR-based technologies could be enhanced with the ability to control the spatial and temporal expression of gRNAs. Here the authors design a tRNA scaffold for highly specific gRNA production from a Pol II promoter.
Databáze: Directory of Open Access Journals