| Autor: |
N. Yang, X. L. Yue, H. Zhang, G. F. Wu, F. X. Ding, T. G. Zhang, L. Z. An |
| Jazyk: |
angličtina |
| Rok vydání: |
2013 |
| Předmět: |
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| Zdroj: |
Biologia Plantarum, Vol 57, Iss 1, Pp 113-120 (2013) |
| Druh dokumentu: |
article |
| ISSN: |
1573-8264 |
| DOI: |
10.1007/s10535-012-0259-z |
| Popis: |
The influence of freezing on phospholipase D (PLD) was studied in Chorispora bungeana Fisch. & C.A. Mey., which is a naturally cold-tolerant species. During the freezing treatment (-4 °C), PLD activities in both microsomal and mitochondrial membranes increased at day 3, remained at a high level at day 6 and then declined to a moderate level. The RT-PCR analyses showed that PLD activity partially corresponded to the CbPLD gene transcript level. The freezing treatment resulted in increases in the K m and V max for microsomal and mitochondrial PLD, respectively. Freezing injury, as measured by electrolyte leakage and malondialdehyde content, peaked at day 6 and then gradually decreased. Alleviation of freezing injury was related to a decreased content of membrane-associated Ca2+. We suggest that the specific mechanism of cold resistance of C. bungeana is linked with PLD. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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