Study the effect of cloned pET-32a(+) plasmid by Lysostaphin gene against Staphylococcus aureus

Autor: Jalal Mustafa
Jazyk: Arabic<br />English
Rok vydání: 2020
Předmět:
Zdroj: Iraqi Journal of Veterinary Sciences, Vol 35, Iss 2, Pp 233-238 (2020)
Druh dokumentu: article
ISSN: 1607-3894
2071-1255
DOI: 10.33899/ijvs.2020.126698.1362
Popis: Lysostaphin is a protein zinc metalloproteinase, extracted from Staphylococcus simulans, which disrupting peptide layer of S. aureus. In this study, Lysostaphin gene was detected in the S. simulans isolates. The molecular weight of the Lysostaphin gene is 750 bp. We were used the pET-32a(+) plasmid to cloning lysostaphin gene which transformed to competent rubidium chloride E. coli DH5α for producing the lysostaphin protein. The lysostaphin protein from this gene which isolated from S. simulans, then used the expression of used to killed S. aureus, which has the thick layer of wall that is the very difficult bacteria response to treatment. The result was reported succeeded pET-32a (+) plasmid to expressed lysostaphin gene and gave lysostaphin protein with high quality and quantity. As well as the result was appeared the high accuracy of his tag method in protein extraction and purification, and the quality and quantity more than other studies.
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