| Autor: |
Junyan Shi, William S. Phipps, Benjamin Y. Owusu, Clark M. Henderson, Thomas J. Laha, Jessica O. Becker, Morteza Razavi, Michelle A. Emrick, Andrew N. Hoofnagle |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Journal of Mass Spectrometry and Advances in the Clinical Lab, Vol 26, Iss , Pp 28-33 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2667-145X |
| DOI: |
10.1016/j.jmsacl.2022.09.005 |
| Popis: |
Background: Despite its clear advantages over immunoassay-based testing, the measurement of serum thyroglobulin by mass spectrometry remains limited to a handful of institutions. Slow adoption by clinical laboratories could reflect limited accessibility to existing methods that have sensitivity comparable to modern immunoassays, as well as a lack of tools for calibration and assay harmonization. Methods: We developed and validated a liquid chromatography-tandem mass spectrometry-based assay for the quantification of serum thyroglobulin. The protocol combined peptide immunoaffinity purification using a commercially available, well-characterized monoclonal antibody and mobile phase modification with dimethylsulfoxide (DMSO) for enhanced sensitivity. To facilitate harmonization with other laboratories, we developed a novel, serum-based 5-point distributable reference material (Husky Ref). Results: The assay demonstrated a lower limit of quantification of 0.15 ng/mL ( |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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Full Text from ScienceDirect