| Autor: |
Georgii Konoplev, Artur Kuznetsov, Aleksandr Frorip, Alar Sünter, Vadim Korsakov, Oksana Stepanova, Natalia Roschina, Nikolay Ovsyannikov, Roman Gerasimchuk, Alina Isachkina, Zarina Rustamova, Alena Pavshukova |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Engineering Proceedings, Vol 35, Iss 1, p 25 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2673-4591 |
| DOI: |
10.3390/IECB2023-14595 |
| Popis: |
A novel simple optical sensor based on fast protein liquid chromatography was developed and tested for monitoring end stage renal disease (ESRD) patients treated with continuous ambulatory peritoneal dialysis (CAPD). The device provides direct determination of proteins and lower molecular weight metabolites in effluent peritoneal dialysate using ultraviolet (UV) photometric detection at the wavelengths 285 nm or 260 nm with deep ultraviolet light-emitting diodes. The sensor was calibrated with bovine serum albumin and nucleotides standard solutions. Chromatograms of peritoneal dialysate samples taken from a group of 28 ESRD patients were processed and approximated by a set of split-Gaussian functions. All chromatograms show three overlapping peaks: the first one represents proteins; the other two peaks probably correspond to mid- and low molecular weight metabolites. Strong correlation was reveled between the area of the first peak and total protein concentration determined by a standard biochemical assay, this makes possible estimation of peritoneal protein loss with a reasonable precision less than 15%. The area of the second peak correlated with dialysate optical density at a wavelength 355–365 nm, associated with the UV absorption of advanced glycation end (AGE) products. The third peak correlated with the optical density of the eluate at a wavelength 255–265 nm, associated with the UV absorption of purines and pyrimidines. Thus, we demonstrated the possibility of estimation of proteins and lower molecular weight metabolites in effluent peritoneal dialysate with the compact and affordable chromatographic optical sensor. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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