Essential Oil Variability of Azorean Cryptomeria japonica Leaves under Different Distillation Methods, Part 2: Molluscicidal Activity and Brine Shrimp Lethality

Autor: Filipe Arruda, Ana Lima, Luísa Oliveira, Tânia Rodrigues, Alexandre Janeiro, José S. Rosa, Elisabete Lima
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Separations, Vol 10, Iss 4, p 241 (2023)
Druh dokumentu: article
ISSN: 2297-8739
DOI: 10.3390/separations10040241
Popis: This study reports on the in vivo molluscicidal activity and Artemia salina lethality of Azorean Cryptomeria japonica leaf (CJL) essential oils (EOs) obtained by hydrodistillation (HD) and water-steam distillation (WSD) techniques, especially in light of the recent focus on the use of forestry and wood industry residues in a sustainable world economy. Molluscicidal activity was performed during several life stages of Radix peregra by the immersion method, under laboratory conditions. A first screening through a single-dose bioassay revealed that both EOs were highly active towards eggs, juveniles and adult snails (ca. 100% mortality). Concentration- and time-toxicity assays were carried out only on adult snails to determine the lethal parameters (LC50;90 and LT50;90). The LC50 values were 33 and 62 µg/mL for EO–WSD and EO–HD, respectively, after 48 h. The LT50 of EO–WSD required only 21 h for both 16 h and continous exposure periods, while that of EO–HD was slighty superior (21.8–25.6 h). Although not significant, EO–WSD was also slightly more toxic against A. salina than EO–HD (LC50 = 98 and 115 µg/mL after 24 h, respectively). In conclusion, Azorean CJL EOs, which are rich in α-pinene (mostly EO–WSD), have huge potential to be used as safe raw materials for the development of natural molluscicide products to control snails responsible for transmitting fascioliasis.
Databáze: Directory of Open Access Journals
Nepřihlášeným uživatelům se plný text nezobrazuje