Cofilin-mediated actin filament network flexibility facilitates 2D to 3D actomyosin shape change

Autor: Zachary Gao Sun, Vikrant Yadav, Sorosh Amiri, Wenxiang Cao, Enrique M. De La Cruz, Michael Murrell
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: European Journal of Cell Biology, Vol 103, Iss 1, Pp 151379- (2024)
Druh dokumentu: article
ISSN: 0171-9335
83212949
DOI: 10.1016/j.ejcb.2023.151379
Popis: The organization of actin filaments (F-actin) into crosslinked networks determines the transmission of mechanical stresses within the cytoskeleton and subsequent changes in cell and tissue shape. Principally mediated by proteins such as α-actinin, F-actin crosslinking increases both network connectivity and rigidity, thereby facilitating stress transmission at low crosslinking yet attenuating transmission at high crosslinker concentration. Here, we engineer a two-dimensional model of the actomyosin cytoskeleton, in which myosin-induced mechanical stresses are controlled by light. We alter the extent of F-actin crosslinking by the introduction of oligomerized cofilin. At pH 6.5, F-actin severing by cofilin is weak, but cofilin bundles and crosslinks filaments. Given its effect of lowering the F-actin bending stiffness, cofilin- crosslinked networks are significantly more flexible and softer in bending than networks crosslinked by α-actinin. Thus, upon local activation of myosin-induced contractile stress, the network bends out-of-plane in contrast to the in-plane compression as observed with networks crosslinked by α-actinin. Here, we demonstrate that local effects on filament mechanics by cofilin introduces novel large-scale network material properties that enable the sculpting of complex shapes in the cell cytoskeleton.
Databáze: Directory of Open Access Journals
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