Identification of Gut Bacteria such as Lactobacillus johnsonii that Disseminate to Systemic Tissues of Wild Type and MyD88–/– Mice
| Autor: | Sreeram Udayan, Panagiota Stamou, Fiona Crispie, Ana Hickey, Alexandria N. Floyd, Chyi-Song Hsieh, Paul D. Cotter, Orla O’Sullivan, Silvia Melgar, Paul W. O’Toole, Rodney D. Newberry, Valerio Rossini, Ken Nally |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Gut Microbes, Vol 14, Iss 1 (2022) |
| Druh dokumentu: | article |
| ISSN: | 1949-0976 1949-0984 19490976 |
| DOI: | 10.1080/19490976.2021.2007743 |
| Popis: | In healthy hosts the gut microbiota is restricted to gut tissues by several barriers some of which require MyD88-dependent innate immune sensor pathways. Nevertheless, some gut taxa have been reported to disseminate to systemic tissues. However, the extent to which this normally occurs during homeostasis in healthy organisms is still unknown. In this study, we recovered viable gut bacteria from systemic tissues of healthy wild type (WT) and MyD88−/− mice. Shotgun metagenomic-sequencing revealed a marked increase in the relative abundance of L. johnsonii in intestinal tissues of MyD88−/− mice compared to WT mice. Lactobacillus johnsonii was detected most frequently from multiple systemic tissues and at higher levels in MyD88−/− mice compared to WT mice. Viable L. johnsonii strains were recovered from different cell types sorted from intestinal and systemic tissues of WT and MyD88−/− mice. L. johnsonii could persist in dendritic cells and may represent murine immunomodulatory endosymbionts. |
| Databáze: | Directory of Open Access Journals |
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