| Autor: |
N. N. Petrishchev, T. G. Grishacheva, S. G. Chefu |
| Jazyk: |
ruština |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Biomedical Photonics, Vol 11, Iss 1, Pp 20-26 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2413-9432 |
| DOI: |
10.24931/2413-9432-2022-11-1-20-26 |
| Popis: |
The goal of the study is to comparatively examine the effect of photoactivated rose bengal on platelet aggregation in vitro and in circulating blood of male Wistar rats. Platelet-rich plasma (PRP) was obtained from venous blood. The aggregation activity of platelets was determined by the turbidimetric method, the aggregation inducer was ADP at a final concentration of 1.25 μM. Rose bengal (RB) (Acros Organics, USA) was used as a photosensitizer (PS). PRP samples containing the PS were irradiated using ALOD-Izumrud laser (OOO “Alcom Medica”, Russia), λ = 532 nm, power density 0.05 W/cm2 , energy density of 6, 12 and 24 J/cm2 . The effect of photoactivated RB on the aggregation of circulating PLT was studied after laser irradiation of the femoral artery of the rats: 30 mW laser power, 2 mm spot diameter and 30 min exposure. RB at concentrations of 0.5 and 1 μg/ml was found to stimulate, and 5-10 μg/ml—to inhibit platelet aggregation. Photoactivation of RB weakens the stimulating effect of laser irradiation on the aggregation of platelets. Photodynamic modification of blood led to an increase in the in[1]tensity of platelet aggregation by 24% in comparison to the control group, and by 39.6% compared to the group without photoactivation of RB (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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