Study of Autoantibodies and DQ Antigens in Patient with Celiac Disease
| Autor: | Andleeb Zehra, Usha, Richa Katiyar, Shailja Singh, Anju Bharti, Neeraj Kumar Agrawal, Om Prakash Mishra, Mohan Kumar |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Journal of Clinical and Diagnostic Research, Vol 14, Iss 10, Pp EC11-EC15 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2249-782X 0973-709X |
| DOI: | 10.7860/JCDR/2020/40617.14121 |
| Popis: | Introduction: Celiac Disease (CD) is a chronic autoimmune mediated disorder triggered by the ingestion of gluten. It is seen in genetically predisposed person and results in small intestine injury. Its aetiopathogenesis is not clear. Simple histopathology is not able to diagnose the disease many times. Aim: Aim of present study was to assess the prevalence of HLA DQ alleles and autoantibodies in diagnosis of the disease and association of DQ antigens with Type 1 Diabetes mellitus (T1DM) and autoimmune hypothyroidism in CD patients. Materials and Methods: Total 100 cases of CD and 31 healthy controls were studied, within a period of January 2015 to Febuary 2016. Autoantibodies like ANA, anti-tTg, anti-TPO and anti-scl 70 were done by ELISA kits. HLA DQ typing was done in 44 cases of CD, 20 cases of CD with T1DM, 22 cases of CD with autoimmune thyroid disease and 31 healthy controls. HLA DQ typing was done by SSO hybridisation method by Mr. SPOT machine. Results: About 70% patients were children between 6 months to 20 years of age and female formed the maximum number of cases (60%). Anti-tTg ab was positive in all cases (100%), anti-Scl 70 Ab was positive in 25%, anti-TPO ab was found in 22% and ANA was positive in only 10% cases. Most frequent DQβ1 haplotype in CD were DQβ1*02:01 (45.5%, p |
| Databáze: | Directory of Open Access Journals |
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