High throughput screening of the potential biosurfactants production by extremophiles isolated from vinasse and black liquor

Autor: Mariano Rivero, Dolores Gutiérrez-Cacciabue, Diego Gastón Sanguino-Jorquera, Verónica Beatriz Rajal, Verónica Patricia Irazusta
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Results in Engineering, Vol 15, Iss , Pp 100587- (2022)
Druh dokumentu: article
ISSN: 2590-1230
DOI: 10.1016/j.rineng.2022.100587
Popis: The aims of this work were to isolate microorganisms from vinasse (V) and black liquor (BL), by-products of alcohol and paper industries and to assess their potential as substrates to produce biosurfactants. Thirty-nine microorganisms, 19 from V (acidophilic, a1 to a19) and 20 from BL (alkaliphilic, b1 to b20) were isolated. A high- throughput method was design and set up for surface tension (γ) measurement. In the first screening, 15/39 strains were pre-selected for their capacity of γ reduction of media formulated with V and BL. A Student's t-test was applied to data obtained in the second screening, to search for significant differences among γ values reached in each production media and their respective sterile medium. Three acidophilic (a1, a5, a6) and 3 alkaliphilic (b1, b2, b17) significantly decreased the γ compared to the control (p
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