Development diagnostic methods of bacterial blight of rice on based PCR
| Autor: | M S Egorova, A N Ignatov, E S Mazurin |
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| Jazyk: | English<br />Russian |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | RUDN Journal of Agronomy and Animal Industries, Vol 0, Iss 2, Pp 22-27 (2014) |
| Druh dokumentu: | article |
| ISSN: | 2312-797X 2312-7988 |
| DOI: | 10.22363/2312-797X-2014-2-22-27 |
| Popis: | Primers identifying bacterial blight pathogen, without cross-reactions with closely related species were designed based on the analysis of the original and GeneBank DNA sequences of Xanthomonas oryzae pv. oryzae. Media YPGA containing cycloheximide 50 mg / l + cefazolin 30 mg / l + gentamicin 2 mg / l has been optimized for BIO-PCR. Sensitivity of the BIO-PCR analysis using original selective media up to 100 times more effective than direct PCR analysis. The sensitivity of the primers in BIO-PCR analysis with original selective media has achieved 50 CFU/ml. |
| Databáze: | Directory of Open Access Journals |
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