Electric cell-substrate impedance sensing in biocompatibility research
| Autor: | Kociubiński Andrzej |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Journal of Electrical Bioimpedance, Vol 12, Iss 1, Pp 163-168 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1891-5469 2021-0019 |
| DOI: | 10.2478/joeb-2021-0019 |
| Popis: | In this paper, the possibility of using cell culture impedance measurements to assess the biocompatibility of a material in contact with cells was analyzed. For this purpose, the Electric Cell-substrate Impedance Sensing (ECIS) method and a commercial measuring device were used. The test substrates with thin-film electrodes made of various metals were prepared using the magnetron sputtering method. The choice of metals was dictated by their varying degrees of biocompatibility. Cultures of mouse fibroblasts were cultured on the prepared substrates. The experiment showed that the complete cycle of culture from attachment and reproduction to apoptosis occurred. The results obtained indicate that it is possible to use the ECIS method to study the influence of metal on cell culture activity. |
| Databáze: | Directory of Open Access Journals |
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