Multiplexed base editing through Cas12a variant-mediated cytosine and adenine base editors

Autor: Fangbing Chen, Meng Lian, Bingxiu Ma, Shixue Gou, Xian Luo, Kaiming Yang, Hui Shi, Jingke Xie, Weika Ge, Zhen Ouyang, Chengdan Lai, Nan Li, Quanjun Zhang, Qin Jin, Yanhui Liang, Tao Chen, Jiaowei Wang, Xiaozhu Zhao, Lei Li, Manya Yu, Yinghua Ye, Kepin Wang, Han Wu, Liangxue Lai
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Communications Biology, Vol 5, Iss 1, Pp 1-12 (2022)
Druh dokumentu: article
ISSN: 2399-3642
DOI: 10.1038/s42003-022-04152-8
Popis: Cas12a-mediated cytosine (CBE) and adenine base editor (ABE) systems with elevated efficiencies and expanded target scopes are developed, by combining highly active deaminases with Cas12a variants, enCas12a, RR and RVR, recognizing non-TTTV PAMs.
Databáze: Directory of Open Access Journals
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