A feasible in vitro method to evaluate bacterial infiltration in three implant-abutment connection systems

Autor: Alexandre Cavalcante de Queiroz, Ivana Barbosa Suffredini, José Dimas de Oliveira, Jefferson de Souza Silva, Eduardo Fernandes Bondan
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Oral Research, Vol 13, Iss 1, Pp 26-36 (2024)
Druh dokumentu: article
ISSN: 0719-2460
0719-2479
DOI: 10.17126/joralres.2024.003
Popis: Introduction: Microorganism infiltration through the im-plant-abutment interface causes oral health problems such as periimplantitis, leading to implant loss. Materials and Methods: A feasible new method to quantify the Streptococcus mutans (S. mutans) infiltration through the implant-abutment interface gap is introduced in the present work. Internal hexagon (IH; n = 10), external hexagon (EH; n = 10), Morse taper (MT; n = 10), and a control for each group (n = 1) were tested. Bacteria suspension was prepared at 1.5x108 CFU/mL (CFU: colony forming units), and the implants were individually submerged up to the connection level, allowing the bacteria to contact it. The abutment was removed, and bacteria count was performed. Results: The implant sets were tested under normal bacterial growth and early and late biofilm growth conditions. Colony-forming units per mL were obtained, and the results were compared among groups. Differences in bacterial count between the MT and EH (p
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