| Autor: |
Xiao Liu, Kevin Lam, Huayi Zhao, Sadatsugu Sakane, Hyun Young Kim, Alvaro Eguileor, Karin Diggle, Shuai Wu, Raquel Carvalho Gontijo Weber, Pejman Soroosh, Mojgan Hosseini, Kristin Mekeel, David A. Brenner, Tatiana Kisseleva |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 4, Iss 3, Pp 102391- (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2023.102391 |
| Popis: |
Summary: Here, we present a protocol for isolating human hepatocytes and neural progenitor cells from normal and nonalcoholic steatohepatitis livers. We describe steps for perfusion for scaled-up liver cell isolation and optimization of chemical digestion to achieve maximal yield and cell viability. We then detail a liver cell cryopreservation and potential applications, such as the use of human liver cells as a tool to link experimental and translational research. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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