Biological Parameters and Molecular Markers of Clone CL Brener - The Reference Organism of the Trypanosoma cruzi Genome Project
| Autor: | Bianca Zingales, Maria Elizabeth S Pereira, Kátia A Almeida, Eufrosina S Umezawa, Nédia S Nehme, Riva P Oliveira, Andrea Macedo, Ricardo P Souto |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 1997 |
| Předmět: | |
| Zdroj: | Memorias do Instituto Oswaldo Cruz, Vol 92, Iss 6, p 811 (1997) |
| Druh dokumentu: | article |
| ISSN: | 0074-0276 1678-8060 |
| DOI: | 10.1590/S0074-02761997000600016 |
| Popis: | Clone CL Brener is the reference organism used in the Trypanosoma cruzi Genome Project. Some biological parameters of CL Brener were determined: (a) the doubling time of epimastigote forms cultured in liver infusion-tryptose (LIT) medium at 28oC is 58±13 hr; (b) differentiation of epimastigotes to metacyclic trypomastigotes is obtained by incubation in LIT-20% Grace´s medium; (c) trypomastigotes infect mammalian cultured cells and perform the complete intracellular cycle at 33 and 37oC; (d) blood forms are highly infective to mice; (e) blood forms are susceptible to nifurtimox and benznidazole. The molecular typing of CL Brener has been determined: (a) isoenzymatic profiles are characteristic of zymodeme ZB; (b) PCR amplification of a 24Sa ribosomal RNA sequence indicates it belongs to T. cruzi lineage 1; (c) schizodeme, randomly amplified polymorphic DNA (RAPD) and DNA fingerprinting analyses were performed |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|