Formulation of Neem oil-loaded solid lipid nanoparticles and evaluation of its anti-Toxoplasma activity
| Autor: | Sara Nemati, Hanieh Mohammad Rahimi, Zahra Hesari, Meysam Sharifdini, Nooshin Jalilzadeh Aghdam, Hamed Mirjalali, Mohammad Reza Zali |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | BMC Complementary Medicine and Therapies, Vol 22, Iss 1, Pp 1-11 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2662-7671 60874686 |
| DOI: | 10.1186/s12906-022-03607-z |
| Popis: | Abstract Background Toxoplasmosis is caused by an intracellular zoonotic protozoan, Toxoplasma gondii, which could be lethal in immunocompromised patients. This study aimed to synthesize Neem oil-loaded solid lipid nanoparticles (NeO-SLNs) and to evaluate the anti-Toxoplasma activity of this component. Methods The NeO-SLNs were constructed using double emulsification method, and their shape and size distribution were evaluated using transmission electron microscope (TEM) and dynamic light scattering (DLS), respectively. An MTT assay was employed to evaluate the cell toxicity of the component. The anti-Toxoplasma activity of NeO-SLNs was investigated using vital (trypan-blue) staining. Anti-intracellular Toxoplasma activity of NeO-SLNs was evaluated in T. gondii-infected Vero cells. Results The TEM analysis represented round shape NeO-SLNs with clear and stable margins. DLS analysis showed a mean particle size 337.6 nm for SLNs, and most of nanoparticles were in range 30 to 120 nm. The cell toxicity of NeO-SLNs was directly correlated with the concentration of the component (P-value = 0.0013). The concentration of NeO-SLNs, which was toxic for at least 50% of alive T. gondii (cytotoxic concentration (CC50)), was > 10 mg/mL. The ability of NeO-SLNs to kill Toxoplasma was concentration-dependent (P-value |
| Databáze: | Directory of Open Access Journals |
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