Transgenic Efficiency of FoxN1-targeted Pig Parthenogenetic Embryos

Autor:	Jae-Hoon Yeo, In-Sul Hwang, Jae Kyung Park, Dae-Jin Kwon, Seoki Im, Eung-Woo Park, Jeong-Woong Lee, Choon-Keun Park, Seongsoo Hwang
Jazyk:	English<br />Korean
Rok vydání:	2014
Předmět:	crispr/cas9 system foxn1 pig parthenogenetic embryos Biotechnology TP248.13-248.65 Medicine (General) R5-920 Internal medicine RC31-1245
Zdroj:	Journal of Animal Reproduction and Biotechnology, Vol 29, Iss 4, Pp 339-344 (2014)
Druh dokumentu:	article
ISSN:	2671-4639 2671-4663
DOI:	10.12750/JET.2014.29.4.339
Popis:	The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR associated protein (Cas9) system can be applied to produce transgenic pigs. Therefore, we applied CRISPR/Cas9 system to generate FoxN1-targeted pig parthenogenetic embryos. Using single guided RNA targeted to pig FoxN1 genes was injected into cytoplasm of in vitro matured oocyte before electrical activation. In results, regardless of the concentrations of vector, the cleavage rate were significantly (p
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/ae067ab4a69643e29cc62d61d7fc3ebf Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF