Characterization of human islet function in a convection‐driven intravascular bioartificial pancreas

Autor: Ana G. Santandreu, Parsa Taheri‐Tehrani, Benjamin Feinberg, Alonso Torres, Charles Blaha, Rebecca Shaheen, Jarrett Moyer, Nathan Wright, Gregory L. Szot, William H. Fissell, Shant Vartanian, Andrew Posselt, Shuvo Roy
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Bioengineering & Translational Medicine, Vol 8, Iss 2, Pp n/a-n/a (2023)
Druh dokumentu: article
ISSN: 2380-6761
DOI: 10.1002/btm2.10444
Popis: Abstract Clinical islet transplantation for treatment of type 1 diabetes (T1D) is limited by the shortage of pancreas donors and need for lifelong immunosuppressive therapy. A convection‐driven intravascular bioartificial pancreas (iBAP) based on highly permeable, yet immunologically protective, silicon nanopore membranes (SNM) holds promise to sustain islet function without the need for immunosuppressants. Here, we investigate short‐term functionality of encapsulated human islets in an iBAP prototype. Using the finite element method (FEM), we calculated predicted oxygen profiles within islet scaffolds at normalized perifusion rates of 14–200 nl/min/IEQ. The modeling showed the need for minimum in vitro and in vivo islet perifusion rates of 28 and 100 nl/min/IEQ, respectively to support metabolic insulin production requirements in the iBAP. In vitro glucose‐stimulated insulin secretion (GSIS) profiles revealed a first‐phase response time of
Databáze: Directory of Open Access Journals
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