Tissue-engineered composite tracheal grafts create mechanically stable and biocompatible airway replacements

Autor: Lumei Liu, Sayali Dharmadhikari, Barak M Spector, Zheng Hong Tan, Catherine E Van Curen, Riddhima Agarwal, Sarah Nyirjesy, Kimberly Shontz, Sarah A Sperber, Christopher K Breuer, Kai Zhao, Susan D Reynolds, Amy Manning, Kyle K VanKoevering, Tendy Chiang
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Journal of Tissue Engineering, Vol 13 (2022)
Druh dokumentu: article
ISSN: 2041-7314
20417314
DOI: 10.1177/20417314221108791
Popis: We tested composite tracheal grafts (CTG) composed of a partially decellularized tracheal graft (PDTG) combined with a 3-dimensional (3D)-printed airway splint for use in long-segment airway reconstruction. CTG is designed to recapitulate the 3D extracellular matrix of the trachea with stable mechanical properties imparted from the extraluminal airway splint. We performed segmental orthotopic tracheal replacement in a mouse microsurgical model. MicroCT was used to measure graft patency. Tracheal neotissue formation was quantified histologically. Airflow dynamic properties were analyzed using computational fluid dynamics. We found that CTG are easily implanted and did not result in vascular erosion, tracheal injury, or inflammation. Graft epithelialization and endothelialization were comparable with CTG to control. Tracheal collapse was absent with CTG. Composite tracheal scaffolds combine biocompatible synthetic support with PDTG, supporting the regeneration of host epithelium while maintaining graft structure.
Databáze: Directory of Open Access Journals
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