Autor: |
Ilya Tsukalov, Ildefonso Sánchez-Cerrillo, Olga Rajas, Elena Avalos, Gorane Iturricastillo, Laura Esparcia, María José Buzón, Meritxell Genescà, Camila Scagnetti, Olga Popova, Noa Martin-Cófreces, Marta Calvet-Mirabent, Ana Marcos-Jimenez, Pedro Martínez-Fleta, Cristina Delgado-Arévalo, Ignacio de los Santos, Cecilia Muñoz-Calleja, María José Calzada, Isidoro González Álvaro, José Palacios-Calvo, Arantzazu Alfranca, Julio Ancochea, Francisco Sánchez-Madrid, Enrique Martin-Gayo |
Jazyk: |
angličtina |
Rok vydání: |
2024 |
Předmět: |
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Zdroj: |
Nature Communications, Vol 15, Iss 1, Pp 1-18 (2024) |
Druh dokumentu: |
article |
ISSN: |
2041-1723 |
DOI: |
10.1038/s41467-024-46322-8 |
Popis: |
Abstract Increased recruitment of transitional and non-classical monocytes in the lung during SARS-CoV-2 infection is associated with COVID-19 severity. However, whether specific innate sensors mediate the activation or differentiation of monocytes in response to different SARS-CoV-2 proteins remain poorly characterized. Here, we show that SARS-CoV-2 Spike 1 but not nucleoprotein induce differentiation of monocytes into transitional or non-classical subsets from both peripheral blood and COVID-19 bronchoalveolar lavage samples in a NFκB-dependent manner, but this process does not require inflammasome activation. However, NLRP3 and NLRC4 differentially regulated CD86 expression in monocytes in response to Spike 1 and Nucleoprotein, respectively. Moreover, monocytes exposed to Spike 1 induce significantly higher proportions of Th1 and Th17 CD4 + T cells. In contrast, monocytes exposed to Nucleoprotein reduce the degranulation of CD8 + T cells from severe COVID-19 patients. Our study provides insights in the differential impact of innate sensors in regulating monocytes in response to different SARS-CoV-2 proteins, which might be useful to better understand COVID-19 immunopathology and identify therapeutic targets. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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