Biosynthesis of copper oxide nanoparticles and its therapeutic efficacy against colon cancer

Autor: Tabrez Shams, Khan Azhar U., Mirza Ahmed A., Suhail Mohd, Jabir Nasimudeen R., Zughaibi Torki A., Alam Mahboob
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Nanotechnology Reviews, Vol 11, Iss 1, Pp 1322-1331 (2022)
Druh dokumentu: article
ISSN: 2191-9097
2022-0081
DOI: 10.1515/ntrev-2022-0081
Popis: In the present study, pumpkin seed extract was used to synthesize copper oxide nanoparticles (CuO NPs) along with evaluating its anticancer activity using different molecular biology tools in the human colorectal cancer cell line (HCT-116). Morphological and structural properties of the biogenically synthesized CuO NPs were characterized by UV-visible spectrophotometry (UV-vis), energy-dispersive X-ray spectroscopy (EDS), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). For estimating the anticancer efficacy, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide cytotoxicity, morphological alteration, reactive oxygen species (ROS) formation, and alterations in the mitochondrial membrane potential (MMP) were determined. SEM and TEM data revealed the formation of spherical nanoparticles possessing an average size of 20 nm. The CuO NPs showed 50% inhibitory concentration (IC50) at 25 µg/mL against the HCT-116 cell line. The treatment with IC50 concentration of CuO NPs showed significant shrinking, detachment, membrane blebbing, and shape distortion of cancer cells. Similarly, the IC50 dose of CuO NPs showed significantly early apoptosis in cancer cells compared to late apoptosis. The cancer cell line also showed a dose-dependent increase and decrease in ROS formation and MMP, respectively. The results obtained through various assays indicated significant anticancer efficacy of biogenically synthesized CuO NPs. Thus, further studies are recommended to validate our results using ex vivo and in vivo models.
Databáze: Directory of Open Access Journals
Externí odkaz: