COMPARISON OF DIAGNOSTIC EFFECTIVENESS OF METHODS OF DETECTION OF COXIELLA BURNETII IN BLOOD OF PATIENTS WITH Q FEVER BASED ON AMPLIFICATION OF 16S rRNA GENE FRAGMENTS (STANDARD PCR) AND groEL GENE (REALTIME PCR)
| Autor: | Yu. A. Panferova, O. A. Freilikhman, N. K. Tokarevich, S. F. Karpenko, Kh. M. Galimzyanov |
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| Jazyk: | ruština |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Журнал микробиологии, эпидемиологии и иммунобиологии, Vol 93, Iss 3, Pp 70-74 (2016) |
| Druh dokumentu: | article |
| ISSN: | 0372-9311 2686-7613 |
| DOI: | 10.36233/0372-9311-2016-3-70-74 |
| Popis: | Aim. Comparison of diagnostic capabilities of 2 variants of PCR for detection of Coxiella burnetii persistence in dynamics of infectious process in patients with Q fever. Materials and methods. 110 samples of clinical material, obtained from patients with Q fever in an endemic region for this infection (Astrakhan region), were studied. The samples were studied in a standard PCR (marker - 16S rRNA gene fragment) and in real-time PCR (RT-PCR) (marker - groEL gene fragment). Results. Both markers were established to be perspective for detection of C. burnetii DNA in clinical material, and RT-PCR detects positive result including late stages of the disease (illness day 21 - 31). Conclusion. This study is the first Russian publication on comparison on different PCR variants for detection of C. burnetii in blood of Q fever patients in dynamics of the infectious process. |
| Databáze: | Directory of Open Access Journals |
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