Urinary Extracellular Vesicles: Potential Biomarkers of Renal Function in Diabetic Patients
| Autor: | Agnieszka Kamińska, Mark Platt, Joanna Kasprzyk, Beata Kuśnierz-Cabala, Agnieszka Gala-Błądzińska, Olga Woźnicka, Benedykt R. Jany, Franciszek Krok, Wojciech Piekoszewski, Marek Kuźniewski, Ewa Ł. Stępień |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Journal of Diabetes Research, Vol 2016 (2016) |
| Druh dokumentu: | article |
| ISSN: | 2314-6745 2314-6753 |
| DOI: | 10.1155/2016/5741518 |
| Popis: | The aim of this study was to check the relationship between the density of urinary EVs, their size distribution, and the progress of early renal damage in type 2 diabetic patients (DMt2). Patients were enrolled to this study, and glycated hemoglobin (HbA1c) below 7% was a threshold for properly controlled diabetic patients (CD) and poorly controlled diabetic patients (UD). Patients were further divided into two groups: diabetic patients without renal failure (NRF) and with renal failure (RF) according to the Glomerular Filtration Rate. Density and diameter of EVs were determined by Tunable Resistive Pulse Sensing. Additionally, EVs were visualized by means of Transmission and Environmental Scanning Electron Microscopy. Nano-liquid chromatography coupled offline with mass spectrometry (MALDI-TOF-MS/MS) was applied for proteomic analysis. RF had reduced density of EVs compared to NRF. The size distribution study showed that CD had larger EVs (mode) than UD (115 versus 109 nm; p |
| Databáze: | Directory of Open Access Journals |
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