B-type natriuretic peptide (BNP) serum levels in rats after forced repeated swimming stress
| Autor: | Almira Hadžovic-Džuvo, Amina Valjevac, Nesina Avdagić, Orhan Lepara, Asija Zaćiragić, Radivoj Jadrić, Jasmin Alajbegović, Besim Prnjavorac |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Medicinski Glasnik, Vol 8, Iss 1, Pp 121-125 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1840-0132 1840-2445 |
| Popis: | Aim To estimate the effects of forced repeated swimming stress on BNP serum levels in rats. Methods Adult male Wistar rats weighting between 280-330 g were divided into two groups: control group (n =8) and stress group (n =8). Rats in the stress group were exposed to forced swimming stress daily, for 7 days. The rats were forced to swim in plastic tanks (90 cm wide, 120 cm deep) containing tap water (temperature ca. 25°C). The depth of water was 40 cm. Duration of each swimming session progressively increased from 10 minutes on the irst day to 40 minutes on days 6 and 7. Rats were sacriiced and blood was drawn from abdominal aorta for BNP analysis immediately after the last swimming session. B-type natriuretic serum level was determined by ELISA method using RAT BNP-32 kit (Phoenix Pharmaceutical Inc.). Results There was no statistically signiicant difference between mean BNP serum level in the stress group after the swimming period (0.81±0.14 ng/ml) as compared to the unstressed group of rats (0.8 ±0.08ng/ml). After the swimming period mean body weight slightly decreased in the stress group in comparison with values before stress period (296.3 g vs.272.8 g), but this difference was not statistically signiicant. The stress period had no inluence on food intake in the stress rat group. Conclusion The workload consisting of 40-minutes long swimming session is not suficient to provoke BNP release from myocardium in rats. |
| Databáze: | Directory of Open Access Journals |
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