| Autor: |
Jari Nuutila, Ulla Hohenthal, Jarmo Oksi, Päivi Jalava-Karvinen |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
EBioMedicine, Vol 74, Iss , Pp 103724- (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2352-3964 |
| DOI: |
10.1016/j.ebiom.2021.103724 |
| Popis: |
ABSTRACT: Background: A key factor behind the unnecessary use of antibiotics is the lack of rapid and accurate diagnostic tests. In this study, we developed a novel and fast flow cytometric single-tube method to detect bacterial infections within 30 minutes. Methods: Quantitative flow cytometric four-colour analysis of host biomarkers CD35, CD64, CD329, and MHC class I expression on neutrophils and lymphocytes was performed on samples taken from 841 febrile patients with suspected infection. Obtained data was incorporated into the four-colour bacterial infection (FCBI)-index, using the developed bacterial infection algorithm. Findings: In distinguishing between microbiologically confirmed bacterial (n = 193) and viral (n = 291) infections, the FCBI-index method was superior to serum C-reactive protein (CRP) and procalcitonin (PCT). In 269 confirmed viral respiratory tract infections, 43% (95% CI: 37–49%) of the patients had an increased FCBI-index, suggesting probable bacterial coinfection. Interpretation: The proposed FCBI-index test might be a potent additional tool when assessing appropriateness of empiric antibiotic treatment. Funding: This study has been financially supported by Turku University Hospital (Turku, Finland) and The Finnish Medical Foundation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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