Occurrence and diversity of nontuberculous mycobacteria affected by water stagnation in building plumbing

Autor: Iftita Rahmatika, Dai Simazaki, Futoshi Kurisu, Hiroaki Furumai, Ikuro Kasuga
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Water Supply, Vol 23, Iss 12, Pp 5017-5028 (2023)
Druh dokumentu: article
ISSN: 1606-9749
1607-0798
01840290
DOI: 10.2166/ws.2023.318
Popis: Nontuberculous mycobacteria (NTM) refer to mycobacteria other than Mycobacterium tuberculosis and Mycobacterium leprae. Some NTM are known to cause pulmonary and skin diseases. As NTM are prevalent in water supply systems, the health burden of waterborne NTM is concerned. However, little is known about NTM in building plumbing. This study revealed the abundance and diversity of NTM in building plumbing by using culture-dependent and culture-independent approaches. Moreover, the chlorine susceptibility of NTM isolates in tap water was evaluated. The increase in the abundance of Mycobacterium spp. was observed in some taps after 24 h of stagnation, indicating that NTM could have the potential to regrow in building plumbing. While Mycobacterium mucogenicum and Mycobacterium avium were detected by cultivation, amplicon sequencing of NTM-specific hsp65 genes revealed that Mycobacterium gordonae was dominant in most of the samples, which was not detected by cultivation. The chlorine susceptibility experiment demonstrated that NTM strains related to M. avium and M. mucogenicum were 119 and 426 times more tolerant to chlorine than Escherichia coli, respectively. As M. mucogenicum, M. avium, and M. gordonae are regarded as opportunistic pathogens, intensive monitoring of NTM in tap water in building plumbing is necessary. HIGHLIGHTS NTM in building plumbing had the potential to increase after water stagnation.; Potentially pathogenic M. avium and M. mucogenicum were isolated from building plumbing, which were more tolerant to chlorine than E. coli.; Integration of both culture-dependent and culture-independent approaches is necessary for a comprehensive understanding of NTM diversity in building plumbing.;
Databáze: Directory of Open Access Journals
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