DNA methylation analysis identifies key transcription factors involved in mesenchymal stem cell osteogenic differentiation

Autor: Rodolfo Gómez, Matt J. Barter, Ana Alonso-Pérez, Andrew J. Skelton, Carole Proctor, Gabriel Herrero-Beaumont, David A. Young
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Biological Research, Vol 56, Iss 1, Pp 1-17 (2023)
Druh dokumentu: article
ISSN: 0717-6287
15634531
DOI: 10.1186/s40659-023-00417-6
Popis: Abstract Background Knowledge about regulating transcription factors (TFs) for osteoblastogenesis from mesenchymal stem cells (MSCs) is limited. Therefore, we investigated the relationship between genomic regions subject to DNA-methylation changes during osteoblastogenesis and the TFs known to directly interact with these regulatory regions. Results The genome-wide DNA-methylation signature of MSCs differentiated to osteoblasts and adipocytes was determined using the Illumina HumanMethylation450 BeadChip array. During adipogenesis no CpGs passed our test for significant methylation changes. Oppositely, during osteoblastogenesis we identified 2462 differently significantly methylated CpGs (adj. p
Databáze: Directory of Open Access Journals
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