| Autor: |
Christian J. Rajkovic, Jovanna A. Tracz, Trevor DeMordaunt, A. Daniel Davidar, Alexander Perdomo-Pantoja, Brendan F. Judy, Kevin Yang Zhang, Vaughn N. Hernandez, Jessica Lin, Julianna L. Lazzari, Ethan Cottrill, Timothy F. Witham |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
North American Spine Society Journal, Vol 18, Iss , Pp 100323- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2666-5484 |
| DOI: |
10.1016/j.xnsj.2024.100323 |
| Popis: |
Background: Postoperative infection is a complication of spinal fusion surgery resulting in increased patient morbidity. Strategies including intraoperative application of powdered vancomycin have been proposed to reduce the incidence of infection; however, such antimicrobial effects are short-lived. Methods: Instrumentation of the L4–L5 vertebrae was performed mimicking pedicle screw and rod fixation in 30 rats. Titanium instrumentation inoculated with either PBS or 1×105 CFU bioluminescent MRSA, along with biomimetic bone grafts infused with varying concentrations of vancomycin and 125 µg of rhBMP-2 (BioMim-rhBMP-2-VCM) were implanted prior to closure. Infection was quantified during the six-week postoperative period using bioluminescent imaging. Arthrodesis was evaluated using micro-CT. Results: Infected animals receiving a bone graft infused with low-dose (0.18 mg/g) or high-dose vancomycin (0.89 mg/g) both exhibited significantly lower bioluminescent signal over the six-week postoperative period than control animals inoculated with MRSA and implanted with bone grafts lacking vancomycin (p=.019 and p=.007, respectively). Both low and high-dose vancomycin-infused grafts also resulted in a statistically significant reduction in average bioluminescence when compared to control animals (p=.027 and p=.047, respectively), independent of time. MicroCT analysis of animals from each group revealed pseudoarthrosis only in the control group, suggesting a correlation between infection and pseudoarthrosis. MRSA-inoculated control animals also had significantly less bone volume formation on micro-CT than the PBS-inoculated control cohort (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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