| Autor: |
GUO Liping, SU Juan, TIAN Xinyan, LU Li, HU Yarong, QIN Yan |
| Jazyk: |
čínština |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
Jichu yixue yu linchuang, Vol 43, Iss 1, Pp 83-86 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1001-6325 |
| DOI: |
10.16352/j.issn.1001-6325.2023.01.0083 |
| Popis: |
Objective To study the role of osteopontin (OPN) during hepatic ischemia-reperfusion injury (HI/RI) in rats. Methods The rats were divided into sham operation group (sham) and ischemia-reperfusion (I/R) 6, 12 and 24 h groups, with 6 rats in each group. HE staining was used to observe the morphological changes of liver tissue. The levels of aspartate aminotransferase (AST) and alanine aminotransferase (ALT) in plasma, the activity of superoxide dismutase (SOD) and the content of malondialdehyde (MDA) in liver tissue were measured by colorimetric method. The expression of OPN mRNA in liver tissue was determined by RT-qPCR. The content of OPN in liver tissue was determined by ELISA. Results Compared with the sham group, HE staining showed obvious degeneration and necrosis of hepatocytes after I/R, which was most obvious in the I/R 24 h group. The plasma levels of AST and ALT increased, most significantly at I/R 12h(PPPPConclusions OPN is involved in the pathogenesis of HI/RI in rats and is a potential indicator applied in the evaluation of liver injury. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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