| Autor: |
Shunyao Zhu, Xueyuan Liao, Yue Xu, Nazi Zhou, Yingzi Pan, Jinlin Song, Taijing Zheng, Lin Zhang, Liyun Bai, Yu Wang, Xia Zhou, Maling Gou, Jie Tao, Rui Liu |
| Jazyk: |
angličtina |
| Rok vydání: |
2025 |
| Předmět: |
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| Zdroj: |
Bioactive Materials, Vol 43, Iss , Pp 392-405 (2025) |
| Druh dokumentu: |
article |
| ISSN: |
2452-199X |
| DOI: |
10.1016/j.bioactmat.2024.09.033 |
| Popis: |
Digital light processing (DLP)-based bioprinting technology holds immense promise for the advancement of hydrogel constructs in biomedical applications. However, creating high-performance hydrogel constructs with this method is still a challenge, as it requires balancing the physicochemical properties of the matrix while also retaining the cellular activity of the encapsulated cells. Herein, we propose a facile and practical strategy for the 3D bioprinting of high-performance hydrogel constructs through the in-situ birth of stem cell spheroids. The strategy is achieved by loading the cell/dextran microdroplets within gelatin methacryloyl (GelMA) emulsion, where dextran functions as a decoy to capture and aggregate the cells for bioprinting while GelMA enables the mechanical support without losing the structural complexity and fidelity. Post-bioprinting, the leaching of dextran results in a smooth curved surface that promotes in-situ birth of spheroids within hydrogel constructs. This process significant enhances differentiation potential of encapsulated stem cells. As a proof-of-concept, we encapsulate dental pulp stem cells (DPSCs) within hydrogel constructs, showcasing their regenerative capabilities in dentin and neovascular-like structures in vivo. The strategy in our study enables high-performance hydrogel tissue construct fabrication with DLP-based bioprinting, which is anticipated to pave a promising way for diverse biomedical applications. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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