Survey of Viruses Present in Radish Fields in 2014

Autor: Jinsoo Chung, Jae-Yeong Han, Jungkyu Kim, Hyekyoung Ju, Junsu Gong, John Hammond, Hyoun-Sub Lim
Jazyk: English<br />Korean
Rok vydání: 2015
Předmět:
Zdroj: Research in Plant Disease, Vol 21, Iss 3, Pp 235-242 (2015)
Druh dokumentu: article
ISSN: 1598-2262
2233-9191
DOI: 10.5423/RPD.2015.21.3.235
Popis: A 2014 nationwide survey in radish fields investigated the distribution of common viruses and possible emerging viruses. Radish leaves with virus-like symptoms were collected and 108 samples assayed by RT-PCR using specific primers for Radish mosaic virus (RaMV), Cucumber mosaic virus (CMV), and Turnip mosaic virus (TuMV); 47 samples were TuMV positive, and RaMV and CMV were detected in 3 and 2 samples, respectively. No samples showed double infection of TuMV/RaMV, or RaMV/CMV, but two double infections of TuMV/CMV were detected. TuMV isolates were sorted by symptom severity, and three isolates (R007-mild; R041 and R065-severe) selected for BLAST and phylogenetic analysis, which indicated that the coat protein (CP) of these isolates (R007, R041, and R065) have approx. 98-99% homology to a previously reported TuMV isolate. RaMV CP showed approx. 99% homology to a previously reported isolate, and the CMV CP is identical to a previously reported Korean isolate (GenBank : GU327368). Three isolates of TuMV showing different pathogenicity (degree of symptom severity) will be valuable to study determinants of pathogenicity.
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