β-glucosidase from thermophilic fungus Thermoascus crustaceus: production and industrial potential
| Autor: | ANDREZA P. GARBIN, NAYARA F.L. GARCIA, GABRIELA F. CAVALHEIRO, MARIA ALICE SILVESTRE, ANDRÉ RODRIGUES, MARCELO F. DA PAZ, GUSTAVO G. FONSECA, RODRIGO S.R. LEITE |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Anais da Academia Brasileira de Ciências, Vol 93, Iss 1 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1678-2690 0001-3765 |
| DOI: | 10.1590/0001-3765202120191349 |
| Popis: | Abstract Microbial β-glucosidases can be used in several industrial processes, including production of biofuels, functional foods, juices, and beverages. In the present work, production of β-glucosidase by solid state cultivation of the fungus Thermoascus crustaceus in a low-cost cultivation medium (comprising agroindustrial residues) was evaluated. The highest production of β-glucosidase, about 415.1 U/g substrate (or 41.51 U/mL), was obtained by cultivating the fungus in wheat bran with 70% humidity, during 96 h at 40°C. The enzymatic activity was optimum at pH 4.5 and 65°C. β-Glucosidase maintained its catalytic activity when incubated at a pH range of 4.0-8.0 and temperature of 30-55°C. The enzyme was strongly inhibited by glucose; even when the substrate and glucose concentrations were equal, the inhibition was not reversed, suggesting a non-competitive inhibition. In the presence of up to 10% ethanol, β-glucosidase maintained its catalytic activity. In addition to β-glucosidase, the enzymatic extract showed activity of 36 U/g for endoglucanase, 256.2 U/g for xylanase, and 18.2 U/g for β-xylosidase. The results allow to conclude that the fungus T. crustaceus has considerable potential for production of β-glucosidase and xylanase when cultivated in agroindustrial residues, thereby reducing the cost of these biocatalysts. |
| Databáze: | Directory of Open Access Journals |
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