Relative expression of oxytocin receptor gene in buffalo endometrium in late luteal phase and pregnancy stages

Autor: Ankita Dilipkumar Verma, Manjit Panigrahi, Bharat Bhushan, Naseer Ahmad Baba, Sourabh Sulabh, Abdul Sadam, Subhashree Parida, Arvind A. Sonwane, Krisnaswami Narayanan
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Applied Animal Research, Vol 46, Iss 1, Pp 146-149 (2018)
Druh dokumentu: article
ISSN: 0971-2119
0974-1844
09712119
DOI: 10.1080/09712119.2016.1277531
Popis: Molecular level information related to buffalo (Bubalus bubalis) reproduction and related genes is not present at appropriate level. If such exploration is made in the form of comparison between expression of genes is made between non-pregnant and pregnant phase, it may be helpful to aid manipulate the reproduction. Hence, the present study was carried out to reveal mRNA quantitative real time expression of oxytocin receptor (OTR) mRNA. IFN-τ is considered as the substance of maternal recognition of pregnancy and shut down the probable mechanisms which lead to luteolysis. Such mechanism includes shutting down of OTR. Therefore, relative expression of OTR was studied in endometrial tissue of three groups. The groups were non-pregnant late luteal phase, pregnancy stage I (pregnancy of 42 days of pregnancy). With designed primer and GAPDH as house-keeping gene, relative mRNA expression was measured in Real-time PCR. After statistical analysis of results, the gene found to be expressed in all three stages with non-significant difference.
Databáze: Directory of Open Access Journals
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