Genetic Manipulation and Transformation Methods for Aspergillus spp.

Autor:	Ye-Eun Son, Hee-Soo Park
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	crispr/cas protoplast agrobacterium aspergillus homologous recombination Botany QK1-989
Zdroj:	Mycobiology, Vol 49, Iss 2, Pp 95-104 (2021)
Druh dokumentu:	article
ISSN:	1229-8093 2092-9323 12298093
DOI:	10.1080/12298093.2020.1838115
Popis:	Species of the genus Aspergillus have a variety of effects on humans and have been considered industrial cell factories due to their prominent ability for manufacturing several products such as heterologous proteins, secondary metabolites, and organic acids. Scientists are trying to improve fungal strains and re-design metabolic processes through advanced genetic manipulation techniques and gene delivery systems to enhance their industrial efficiency and utility. In this review, we describe the current status of the genetic manipulation techniques and transformation methods for species of the genus Aspergillus. The host strains, selective markers, and experimental materials required for the genetic manipulation and fungal transformation are described in detail. Furthermore, the advantages and disadvantages of these techniques are described.
Databáze:	Directory of Open Access Journals
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