Anti-phenolic glycolipid-I (PGL-I) determination using blood collection on filter paper in leprosy patients
| Autor: | TOMIMORI-YAMASHITA Jane, NGUYEN Thu H., MAEDA Solange M., FLAGEUL Beatrice, ROTTA Osmar, CRUAUD Philippe |
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| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: | |
| Zdroj: | Revista do Instituto de Medicina Tropical de São Paulo, Vol 41, Iss 4, Pp 239-242 (1999) |
| Druh dokumentu: | article |
| ISSN: | 0036-4665 1678-9946 |
| Popis: | The authors studied 70 leprosy patients and 20 normal individuals, comparing the traditional sera collection method and the finger prick blood with the conservation on filter paper for specific antibodies against the native phenolic glycolipid-I (PGL-I) from Mycobacterium leprae. The finger prick blood dried on filter paper was eluated in phosphate buffer saline (PBS) containing 0.5% gelatin. The classical method for native PGL-I was performed for these eluates, and compared with the antibody determination for sera. It was observed that there is a straight correlation comparing these two methods; although the titles found for the eluates were lower than those obtained for serology. This blood collection method could be useful for investigation of new leprosy cases in field, specially in contacts individuals. |
| Databáze: | Directory of Open Access Journals |
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