| Autor: |
Zellmer Sebastian, Schmidt-Heck Wolfgang, Gaunitz Frank, Baldysiak-Figiel Alicja, Guthke Reinhardt, Gebhardt Rolf |
| Jazyk: |
angličtina |
| Rok vydání: |
2005 |
| Předmět: |
|
| Zdroj: |
Journal of Integrative Bioinformatics, Vol 2, Iss 1, Pp 58-73 (2005) |
| Druh dokumentu: |
article |
| ISSN: |
1613-4516 |
| DOI: |
10.1515/jib-2005-15 |
| Popis: |
Wnt/β-catenin signalling plays an important role in zonation of liver parenchyma and in patterning of hepatocyte heterogeneity. A characteristic marker of this heterogeneity is glutamine synthetase, which is expressed only in a subset of pericentrally located hepatocytes. To investigate, whether and how the Wnt/β-catenin signalling pathway is involved a culture of hepatocytes was stimulated by LiCl. This resulted in an increase in the specific GS activity, indicating that the Wnt/β-catenin pathway may participate in regulating GS levels. Affymetrix GeneChip oligonucleotide arrays were used to monitor the gene expression changes during a period from 2 to 24 hours after stimulation by LiCl. Samples from a cultivation without stimulation were used as controls. Based on the gene expression profiles a hypothetic signal transduction network was constructed by a reverse engineering algorithm. The network robustness was tested and the most stable structure was identified. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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