A new multiplexed magnetic capture—Droplet digital PCR tool for monitoring wildlife population health and pathogen surveillance

Autor: Christina M. Tschritter, Peter V. C. de Groot, Marsha Branigan, Markus Dyck, Zhengxin Sun, Stephen C. Lougheed
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Ecology and Evolution, Vol 13, Iss 11, Pp n/a-n/a (2023)
Druh dokumentu: article
ISSN: 2045-7758
DOI: 10.1002/ece3.10655
Popis: Abstract Anthropogenic stressors are exacerbating the emergence and spread of pathogens worldwide. In regions like the Arctic, where ecosystems are particularly susceptible, marked changes are predicted in regional diversity, intensity, and patterns of infectious diseases. To understand such rapidly changing host‐pathogen dynamics and mitigate the impacts of novel pathogens, we need sensitive disease surveillance tools. We developed and validated a novel multiplexed, magnetic capture, and ddPCR tool for the surveillance of multiple pathogens in polar bears, a sentinel species that is considered susceptible to climate change and other stressors with a pan‐Arctic distribution. Through sequence‐specific magnetic capture, we concentrated five target template sequences from three zoonotic bacteria (Erysipelothrix rhusiopathiae, Francisella tularensis, and Mycobacterium tuberculosis complex) and two parasitic (Toxoplasma gondii and Trichinella spp.) pathogens from large quantities (
Databáze: Directory of Open Access Journals